Journal: AIChE journal. American Institute of Chemical Engineers

Article Title: Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing.

doi: 10.1002/aic.17466

Figure Lengend Snippet: FIGURE 2 Dual- and multispecies biofilms of Escherichia coli K361 and probiotics with cell-free produced colicins. (A) Lactococcus lactis (5 106 CFU/ml) and (B) probiotic mixture VSL#3 (5 106 CFU/ml) were seeded together with the K361 strain (5 106 CFU/ml) to form dual-species biofilms in polypropylene culture tubes in LB medium at 37C for 24 h. The biofilms were treated with cell-free produced colicins (3.5 nM) for 1 h. Viable biofilm cells are quantified in colony-forming units per area (CFU/cm2). Error bars indicate the standard deviation from two independent cultures with three replicates

Article Snippet: AmpR, KanR, and StrR indicate ampicillin-, kanamycin-, and streptomycin-resistant, respectively Strains and plasmids Genotype/relevant characteristics Source Strains E. coli K361 W3110 strain, StrR 16 E. coli BL21Star (DE3) F ompT, hsdSB (rB mB ), gal, dcm, rne131 (DE3) Invitrogen E. coli K964 K91 containing pColE2 17 E. coli TG1 Strain containing colicin plasmid 18 E. coli DH5α fhuA2, Δ(argF-lacZ)U169, phoA, glnV44, Φ80 Δ(lacZ) M15, gyrA96, recA1, relA1, endA1, thi-1, hsdR17 NEB E. coli Nissle 1917 ΔdegP Probiotic E. coli (EcN) lacking degP, KanR 19 Lactococcus lactis 11454 Probiotic Lactococcus lactis subsp. Lactis ATCC strain (ATCC® 11454TM) ATCC VSL#3 Probiotic mixture VSL3 Pharma Plasmids pKSJ331 AmpR, ColE1 operon 18 pKSJ167 AmpR, ColE3 operon 20 pJL1-sfgfp KmR, PT7::sfGFP, C-terminal Strep-tag, pY71-sfGFP 21 pJL1-E1 KmR, PT7::colicin E1 and E1 immunity This study pJL1-E2 KmR, PT7::colicin E2 and E2 immunity This study pJL1-E3 KmR, PT7::colicin E3 and E3 immunity This study pBAD-gfp AmpR, ParaBAD::GFP Addgene (#54764) pBAD-empty AmpR This study pBAD-E2 AmpR, ParaBAD::colicin E2 and E2 immunity This study pBAD-E2+lys AmpR, ParaBAD::colicin E2 and E2 immunity, Ptet::E7 lysis, PproB::tetR This study nloaded from https://aiche.onlinelibrary.w iley.com /doi/10.1002/aic.17466 by E gyptian N ational Sti.

Techniques: Probiotics, Produced, Standard Deviation